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The second round of PCR was carried out using the corresponding first round PCR product as a template and running the following PCR programme: initial denaturing at 95°C for 3 min, 30 cycles of 95°C for 15 s, 55°C for 30 s, 72°C for 30 s and an extension cycle of 72°C for 10 min.
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It was tense and typical until he ripped up the template and ran solo.
While he was taking a breather, others — in particular, Gucci Mane, and Lil Wayne's protégé Drake — took his template and ran with it.
Simply Crochet, £4.99 Given the success of Mollie Makes, it's not surprising that the publishers have decided to take that template and run with it.
Diagnostic PCRs were performed using 1 μl of genomic DNA as template, and run on 3% 1× Tris-acetate-EDTA agarose gels.
How Soft Cell did leather-clad S&M pop way before Essex Clearasil kids Depeche Mode took the template and ran with it, becoming a sort of Torture Garden-friendly U2 in the process.
Reactions consisted of QuantiTect SYBR Green RT-PCR Master Mix (Qiagen), 0.5 µM oligonucleotides and 200 ng of first strand cDNA template, and run on a RotorGene 2000 (Corbett).
The nested PCR for LSU rDNA was run for 30 cycles in the first reaction (95°C for 0.5 min, 48°C for 1 min, 72°C for 2 min); for the second PCR an 1 µl aliquot of the first PCR was used as template and run for 35 cycles (95°C for 0.5 min, 52°C for 1 min, 72°C for 1 min).
Remove the card template and run a gathering stitch around this newly marked line.
They write apps that tap his existing templates and run on his machines; he rewards his salespeople for selling the stuff and takes a 15% to 20% cut of every deal.
The PCR reactions were performed using 2× ddPCR Supermix for Probes (no dUTP) (Bio-Rad), 20× ddPCR mut assays (FAM/HEX labeled) (Bio-Rad), 5 U of HindIII restriction enzyme (New England BioLabs, Ipswich, MA, USA), and template DNA and ran according to the manufacturer's instructions.
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