Sentence examples for template and random from inspiring English sources

cDNA was generated with the Takara RNA PCR Kit (AMV) with 500 ng of RNA as template and random 9-mers.

cDNA synthesis was carried out using a cDNA synthesis kit (Applied Biosystems Inc., Foster City, CA, USA) using 1 μg total RNA as the template and random primers.

These short mRNA fragments were used as templates, and random hexamer-primers were used as primers for first-strand cDNA synthesis.

Templates and random hexamers were first incubated at 65°C for 10 min, and reverse transcription was performed at 42°C for 90′; the procedure was stopped by enzyme inactivation at 70°C for 10′.

We argue that this is not an annotation artefact, but most likely results from the end of template and reduced random priming potential in the first strand synthesis step amplification.

Positronium annihilation lifetime spectroscopy reveals that porous MSQ film templated by triblock copolymers (M̄n in the range of ∼5000 12,000 g/mol) have smallest pores and highest percolation threshold compared to those templated by diblock and random copolymers.

Andrew et al. constructed an anatomical network using DTI data and reported that small worldness exhibited a 95%% difference between the widely used AAL template and a 4,000-node 4,000-nodecellation ((sigma _{AAL}=1.9,{random},sigma _{4000}=53.6parcellation.

cDNAs were synthesized using the First Strand cDNA kit (GE Healthcare) using 1 µg total RNA as template and pd(N 6 random hexamer primers.

Total RNA was extracted from homogenized tissue according to the protocol provided in the RNeasy tissue Mini Kit (Qiagen, Hilden, Germany), reverse transcribed using 500 ng RNA as template and oligo-dT and Random Hexamers (Ration 1 2) as primers to obtain cDNA fragments.

For all reactions, no-template controls were run, and random RNA preparations were also subjected to sham reverse transcription to check for the absence of genomic DNA amplification.

RNA was purified and first strand cDNA was produced from 1 µg total RNA template using AMV reverse transcriptase and random primers (Promega) following the manufacturer's instructions.