Sentence examples for template and quantified from inspiring English sources
The PCR products were amplified from the single-stranded cDNA as the template and quantified using a Light Cycler 480 (Roche Diagnostics K.K., Tokyo, Japan) according to the following program: 10 s at 95 °C, followed by 50 cycles of 95 °C for 5 s and 60 °C for 34 s.
All RT-PCRs included no-template negative controls and quantified MERS-CoV transcript as positive control.
Crosslinks were reversed by incubating chromatin at 65°C overnight, and enriched DNA template analyzed by traditional PCR and quantified by real-time PCR (described in PCR analyses).
Template was then amplified, detected, and quantified using SYBR green fluorescence.
A deformable template extracted the gene spots and quantified their expression levels by determining the integrated intensity of each spot after background subtraction.
DNA was pooled from each of the wells in equal proportion, and quantified prior to template library preparation.
A minimum of 1 min of traces were filtered at 1 kHz Bessel and quantified using Clampfit template match (Axon Instruments, Sunnyvale, CA).
All RNA templates were purified on denaturing PAGE and quantified by measuring absorbance at 260 nm.
Bands corresponding to 3Dpol cross-linked to template-primer were visualized by a Fuji FLA-5000 phosphorimandr and quantified using FujiFilm MultiGauge (Stamford, CT).
A relational linear program (RLP) is a declarative LP template defining the objective and the constraints through the logical concepts of objects, relations, and quantified variables.
The library was assessed and quantified using a Bioanalyzer (Agilent Technologies, Palo Alto, California, USA), and then diluted to 8 pM for template preparation using an Ion PGM Template OT2 400 kit (Life Technologies) and enriched.
