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We then sequenced all the genes of WT and rel2 mutant with genomic DNA as template, and only found that rel2 mutant has three sequence mutations in locus Os10g0562700, including a 4-bp deletion at 5'-UTR and two nucleotide substitutions at open reading frame (ORF) region (Fig. 4e).
A nystagmus assessment was documented in 95.6% (834/872) of visits having a nystagmus item template and only 8.8% (54/613) of visits without a nystagmus item template.
Notably, the remaining SMARCAL1 residues, 7 13 and 28 32, were disordered in this model because the algorithm does not generate structural models for residues lacking the template and only 17 residues in the UNG2 peptide are ordered.
In addition, the amplification efficiencies of each amplicons were tested by performing qPCR with a serial-diluted cDNA as the template, and only those whose being between 90 and 110 % were used for gene expression analysis.
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A region may be predicted as full copies of the coding regions of two IS templates, and only the copy with smaller overall E-value will be kept.
For practical usage, the visualization of a class is described in a selected template language, and only the name of the template is stored in the database.
The three K+ uptake systems in the template organisms can only be partially mapped to WH8102 (see Template networks); and only KtrBAE (SYNW2168-2169, 0663), is found in WH8102 through mapping, while the other two systems, Kdp and Kup, could not be mapped (see Table 2).
The TomB homology model was built using Modeller34 starting with YmoB as template structure and only rebuilding the regions surrounding insertions or deletions in the sequence alignment.
In the ligase reaction both probes are hybridized to a present template and ligated only in the case of a perfect match in the discriminating position [ 26].
Recently, the annealing control primer (ACP) system has been developed; this technique provides a primer with annealing specificity to the template and allows only genuine products to be amplified [ 10].
A total of 114 DEGs from patients with serous ovarian cancer stage III were identified using the ACP-based GeneFishing™ PCR system, which uses primers that anneal specifically to the template and allows only genuine products to be amplified.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com