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In this study, we use the amphiphilic lipopolysaccharides, derived from Pseudomonas aeruginosa as imprinting template and obtained an evenly distributed sub-40 nm polymeric nanoparticles by using inverse emulsion method.
The PN used the care-plan template and obtained current physical measures and reviewed recent pathology results.
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This is emphasized by aligning the structures from all the other clan CD families with the caspases (using caspase-7 as a template) and obtaining a measure of structural similarity (PDBeFold [ 66], as described above).
The as-synthesized material was calcined from room temperature to 550°C at a heating rate of 2 6°C/min for 5 h to remove the templates and obtained fine mesoporous SBA-15.
Here, the refined comparative model comprised regions that could not be aligned to any of the templates and obtained unacceptably low scores in all models.
In order to build reliable homology models and analyze the structural context of residues under selective pressures, we used the HHpred web-server (http://toolkit.tuebingen.mpg.de/hhpred) [ 76] to identify suitable templates and obtain their sequence alignment with the target protease protein sequences.
After threading, full atomistic models of the Rev protein were generated from the template and alignment obtained from the threading studies using the MODELLER [29], [30], and NEST software tool incorporated in JACKAL 1.5 [49].
Insight II (2000) Biopolymer module software (Accelrys Software Inc., USA), run on an Indigo 2 Station (Silicon Graphics), was used for superimposing the backbones of the original template and the obtained model without further refinements.
The presence or absence of FCT-specific gene products was assessed by PCR, using the genomic DNA of each strain as template, and the obtained gene patterns were assigned to established FCT-types as described [18], [19].
To confirm TI-NGS assay's variant calls at the discrepant positions with Sanger sequencing, we custom designed template-dependent NGS assays and obtained 100% concordance with the TI-NGS assay.
A correction method previously proposed by our group and obtained with template-based attenuation maps (AM) used MR and ECAT HR+ images.
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