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Strategies such as the use of a data extraction template and multiple coders were used to minimise risks of bias being introduced into the review.
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Here, we report the development of an online Java-based software for virtual PCR on linear or circular DNA templates and multiple primer or probe search from large or small databases.
In Sections 4 and 5 we define two additional problem templates: the Outside Vector Multiplication Template, and the Multiple String Vector Multiplication Template, and describe modifications to the algorithm of Valiant which allow to solve problems that sustain these templates.
Drosophila melanogaster pol γ-β structure was modeled with the software MODELLER, version 9.12 (Sali and Blundell 1993), using the 2G4C A file as template and the multiple sequence alignment (MSA) shown in supplementary figure S2, Supplementary Material online, as a parameter.
Biogem encourages software development best practices by providing templates for documentation and multiple test driven development strategies; such as unit tests, behaviour driven development and a natural language parser for software specification (e.g. Chelimsky et al., 2010).
PCR was performed in a final volume of 10 μL containing 1× HotStarTaq buffer, 3.0 mM Mg2+, 0.3 mM dNTP, 1 U HotStarTaq polymerase (Qiagen Inc. USA), 1 μl DNA template and 1 μl multiple primer set.
The proposed scheme has the properties of non-invertibility, revocability and multiple template independence.
In this respect, large amounts of template DNA were used and multiple amplifications examined, as recommended [ 17].
The app also supports group editing and multiple templates allow you to flow images and text into the app seamlessly.
Given a target and template alignment (multiple templates can be used), the system performs several operations: Builds a multiple sequence alignment of representative sequences for both the target and the template families.
To facilitate quantitative sampling of the viral population, cDNA generation from viral RNA and subsequent PCR reactions used high template volume, low cycle numbers, and multiple replicates that were pooled for sequencing (Fig. S5).
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