Sentence examples for template and increase from inspiring English sources
A further overnight digestion step with 1000 U of EcoRI (R0101M, NEB), which cuts outside the hybrid ligation products, was incorporated prior to reversal of crosslinks to shorten the size of the 3C template and increase the PCR efficiency.
The 5'-tail region is non-complementary to the DNA template and increases the melting temperature of the primer once the tail is incorporated into the PCR product.
The effective combination of multiple algorithms considerably extends the coverage of target sequences by distantly related templates and increases the overall modeling accuracy.
In both locations, G-quadruplex formation was associated with dimerization of the homologous templates and increased rate of primer-strand transfers during reverse transcription, suggesting that in vivo the structure contributes to dimerization of the viral genomes that promotes recombination.
This improved dimension reduction power will allow us to have reduced size feature sets (optimal for template storage) and increased matching speed due to these smaller dimensional features.
In addition, several probe-blocking methods have been introduced in real-time AS-PCR to block amplification of wild-type templates and to increase detection sensitivity and specificity.
Therefore we tested whether LNA can mimic RNA-like features in template-switching reactions and increase the performance of nanoCAGE library preparation.
10.7554/eLife.06535.010 Figure 4. Activation of MYC transcription by i-CDK9 depends on induced transfer of kinase-active P-TEFb from 7SK snRNP to BRD4, binding of the BRD4-P-TEFb complex to acetylated MYC chromatin template, and BRD4-mediated increase in CDK9's catalytic activity and resistance to inhibition.
As the angle increases, remove heat long enough to hold the template against the bend and increase incrementally and accordingly.
These structures may not be obtained without special care; to improve conformal coverage, one has to carefully place the template, control the temperature of the template or the distance between the evaporating source and template to increase the directionality of evaporated species.
It couples nucleotide excision repair with transcription by dislodging inactive RNA polymerase molecules stalled at template DNA lesions and increasing the rate at which the Uvr(A BC excinuclease acts at these sites.
