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Eukaryotic gene expression is regulated by dynamic nuclear signaling events that occur at the chromatin template and include post-translational modification of the histone proteins, via methylation, phosphorylation, acetylation and ubiquitination.
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Largely forgotten now, Business for Sterling set the template, and included some of the key personnel, for the 2016 leave campaign.
ROIs, chosen due to their predilection for damage post TBI, were manually drawn using Analyze 7.0 (http://www.mayo.edu/bir) in MNI125 space using Colin27 [17] as a high resolution, high signal-to-noise template, and included the corpus callosum (genu and splenium), thalamus, midbrain, pons, cerebellar peduncles, and cerebellar cortex (Figure 1).
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The images of grey matter obtained in the structural analyses (see above) were transformed to the MNI-152 template and included as voxel-dependent covariates in the group analyses (Oakes et al., 2007).
Here we remove the envelope due to its comparable performance with STA/LTA and include template matching in the comparison.
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We based our design on the endogenous human miRNA genomic template-strand sequences, and included DNA encoding ∼3 helical turns of the pri-miRNA stem-loop sequence (Fig. 1B), the minimum stem length required for Drosha/Microprocessor processing [14], [15].
For relative quantification, standards were established from serial dilutions of APAF-1, DAPK-1 and PBGD cDNA templates, generated separately and included in each run.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com