Imprinting procedures lead to functional properties of polymeric materials, as the analyte itself is used as a template and generates adapted cavities that are suitable for re-inclusion.
The component traverses a given archetype or template and generates an example RM structure valid to the constraints.
The bacterial RNA polymerase utilizes the 6S RNA as a template and generates short (14- to 20-nt) RNA products that are initiated within the bubble [ 6, 10].
When sequence identity is below 30%, it is difficult to recognize the best template and generate accurate sequence-template alignments, so the resultant models have a wide range of accuracies (Chakravarty et al., 2008; Sanchez et al., 2000).
The primer extension reaction uses PCR products as templates and generates distinct mass signals for competitor and cDNA-derived products.
Focusing on the influence of conformational details in the template structures, we based our study on 8 experimentally determined template structures and generated 120 designs from each.
We used the cycling protocol described above; except for longer amplicons (>500 bp), we extended the annealing/extension step to 2 min. For this assay we ran only one well per template amount and generated copy numbers and 95% Poisson confidence intervals with the QuantaSoft platform.
This task can be broken down into several successive steps: inferring protein sequence from the genomic information, performing a search for a template structure, fitting the query sequence to the template and then generating a 3D model.
In these quantitative analyses, the number of copies of sense and antisense RNA was determined based on the net difference between signal generated from specific cDNA template and that generated from primer-independent cDNA template.
This information was used to fill the template and then generate coherent, informative multi-document summaries.
