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When sequence identity is below 30%, it is difficult to recognize the best template and generate accurate sequence-template alignments, so the resultant models have a wide range of accuracies (Chakravarty et al., 2008; Sanchez et al., 2000).
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Imprinting procedures lead to functional properties of polymeric materials, as the analyte itself is used as a template and generates adapted cavities that are suitable for re-inclusion.
The component traverses a given archetype or template and generates an example RM structure valid to the constraints.
The bacterial RNA polymerase utilizes the 6S RNA as a template and generates short (14- to 20-nt) RNA products that are initiated within the bubble [ 6, 10].
The oligonucleotide sequences for qPCR are listed in additional file 3. The efficiency for each primer pair (E) was determined by running 10-fold serial dilutions (4 dilution series) of Rm1021 genomic DNA as template and generating a standard curve by plotting the log of the dilution factor against the CT value during amplification of each dilution.
The primer extension reaction uses PCR products as templates and generates distinct mass signals for competitor and cDNA-derived products.
Focusing on the influence of conformational details in the template structures, we based our study on 8 experimentally determined template structures and generated 120 designs from each.
This information was used to fill the template and then generate coherent, informative multi-document summaries.
In these quantitative analyses, the number of copies of sense and antisense RNA was determined based on the net difference between signal generated from specific cDNA template and that generated from primer-independent cDNA template.
These vectors were used as a template to PCR amplify and generate N-terminal Flag tagged Rab4a and mutant constructs.
It is to amplify a single DNA template from minimally diluted samples and generate amplicons that are exclusively derived from one template.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com