Note: we also did similar analysis for the U.S. participants (e.g., using age-appropriate Chinese or U.S. template), and found analogous findings.
Consistently, we observed the fidelity of hPolι to be the lowest opposite template base dT followed by dC, dG, and finally dA when replicating the undamaged template and found hPolι to preferentially incorporate dG opposite template dT.
As three-finger toxins are uncommon in viperid venoms, we performed RT-PCR using a separate pool of RNA as template and found five transcripts that encode three-finger toxins [ 23].
We validated some of the failed reactions using genomic DNA as template, and found that most of the failures in which no PCR product had formed could be due to very little or no cDNA present in the source of DNA template.
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Copyediting and typesetting are increasingly algorithmic and automatable, as is design, if you're willing to limit yourself to a few templates and find your own cover image.
Finally, we quantified the efficiency and accuracy of PrimPol activity on damaged DNA templates and find that PrimPol preferentially bypasses 8-oxo-dG in an accurate manner.
We quantified the PrimPol misinsertion frequency on undamaged DNA templates and find that the fidelity of polymerization is indeed comparable to other TLS pols (1 mistake every ∼100 2500 insertion events).
Lysine residues play a role in templating the formation of silica on the fibril surface and, using this library of peptides, we have explored the contributions of lysine as well as arginine to silica templating, and find that sequence plays an important role in determining the physical nature and structure of the resulting nanowires.
In summary, we have synthesized flexible chiral cyclodextrin-based templates, and found that they are as effective as rigid templates for directing the synthesis of porphyrin nanorings.
