Sentence examples for template and does from inspiring English sources

Compared to cloning the natural cDNA sequence by reverse transcriptase PCR (RT-PCR), gene synthesis does not require a template and does not suffer from limitations of resources, which is particularly relevant for rare and endangered species or if the gene is expressed at an extremely low level.

If r r exceeds 1.0 (the basic catalytic activity of the hosting surface), then R acts as a real replicase, i.e., it helps replication, but a parasite with r r < 1.0 acts as a „poison" for surface catalysis: it binds the template and does not even let the mineral surface help the replication process.

Addition of mispaired dNTPs or the complementary rUTP gave a much smaller fluorescence decrease, suggesting that they fail to engage effectively with the 2-AP template and do not progress as far along the reaction pathway as the complementary dTTP.

That is fine as long as you are familiar with your template and do not miss out on obtaining any important information.

In this sense, the synthesis is quite different from the conventional epitaxial growth where the substrates only serve as the templates and do not participate in the reactions.

As mentioned by the reviewer: "… the duplex formed by a small circular template is stiff and does not allow the formation of a duplex longer than half of the length of the circle [ 33]".

The 5′ end of the antisense/transcript strand would dissociate automatically because the duplex formed by a small circular template is stiff and does not allow the formation of a duplex longer than half of the length of the circle ([ 33], and references therein).

If the 3' end of either template is not within the region of similar sequence, then no 3' end will hybridize to the other template and synthesis does not lead to recombination.

These results are suggesting that the re-use of the non-reacted precursor solutions is possible as the surfactant template is completely preserved and does not decompose during hydrothermal treatment at mild hydrothermal condition (100°C).

Compartmentalization of the beads within emulsion droplets is necessary during the ePCR step to ensure that each bead only binds DNA amplified from the single template on its surface, and does not exchange DNA with other beads.

Our implementation extracts JSON or HOT template and the extracted template can be deployed both by Amazon CloudFormation and OpenStack Heat because a template is abstract text information and does not depend on IaaS platform.