Sentence examples for template and directly from inspiring English sources
Based on this observation, it was hypothesized that if conditions were sufficiently optimized, it should be possible to take the pre-amplification pool template and directly perform the barcoding PCR step, without an intermediate second-stage amplification on the Access Array chip.
PCR products were amplified using this primer pair and pCR-TOPOII vector containing Gsdma as a template and were directly self-ligated.
The practice in C. elegans of injecting the repair template and Cas9/sgRNA plasmid directly into the meiotic (oogenic) germline syncytium may help favor HDR.
Metal-meshes are fabricated on quartz substrate by lift-off based on self-cracking template and CVD graphene is directly transferred onto thin metal-meshes to construct the hierarchical graphene/metal-mesh film, by which considerable improvements both in microwave reflection and absorption can be realized in thin metal-meshes.
The cDNA samples were diluted 20x and directly used as templates for qPCR.
The genome with the target gene was used directly as template, and DpnI was unnecessary.
PCR products were gel purified with the QIAquick kit (Qiagen, Valencia, CA, USA) according to the manufacturer's protocol, and the resulting template was directly sequenced with the amplifying primers.
PCR products were gel-purified by using the QIAquick kit (Qiagen), according to the manufacturer's protocol, and the resulting template was directly sequenced by using the amplifying primers.
By coregistering male and female brains to our template, we directly compared labeled clones from both sexes (see Supplemental Experimental Procedures).
The surface of these carbon spheres is hydrophilic and possesses -OH and -C=O groups, which renders the surface of the template microspheres directly workable.
In this study, ion imprinted polymers (IIPs) using cadmium ions as the template were directly grafted on the surface of low-cost print paper based on the reversible addition-fragmentation chain transfer polymerization.
