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A possible mechanism was suggested that biomineralization may occur during the carbonation of calcium hydroxide, where the sticky rice functions as a template and controls the growth of calcium carbonate crystal.
Controls without template and controls without RT reactions were included.
Control reactions lacking template and controls with template but without Reverse Transcriptase were used.
In their view, the matrix acts as a template and controls crystallization within its bounds.
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Negative control without cDNA template and control of genomic DNA contamination in RNA samples were performed for each pair of primers and each batch of RNA extracted.
The templates and controls used here were the same as for qRT-PCR described above.
For quality control of the bead-based MLPA four control samples are run with every MLPA experiment [ 10]: assay control, no template control, contamination control and MAGPIX control.
No template and negative controls containing water-based cDNA were used to rule out component contamination.
No-template controls and controls omitting RT enzyme were included on each assay plate to detect potential DNA contamination.
Control reactions included a no template control and a control without reverse transcriptase.
Each plate included triplicate wells of "no template control" and 4 control samples.
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