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The full-length EZH2 cDNA was obtained by PCR using an EST clone as template and constructed into pCEP4 expression vector to express EZH2 as a Flag-tagged protein.
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The full-length BCL2L2 and E2F6 cDNAs were obtained by PCR using EST clones as templates and constructed into pcDNA3-HA vector for creation of the stable expression cell lines.
Drawing on team members' experiences of managing teams, we have adapted a template shared by PB and constructed a management and research governance framework to support the four phases of our study (1.
(A ) Diagrams of Pol II promoters (CDKN1A, ACTB, RPPH1, and GDF15 ) with locations of exon 1, SINEs (red), and constructed DNA template (green arrow) for the in vitro MAF1 binding assay.
Mutant P particles with single amino acid substitutions were designed and constructed by site-directed mutagenesis using the corresponding wild type constructs as templates.
In that study, SPECT images were not registered with CT or MR images, but a region of interest template was constructed and used to evaluate receptor binding [ 108].
The template is generally constructed and embedded in the cover, and the geometrical parameters are sought via a particular technique.
Yoon et al. (2009) compared the tissue distributions obtained from normalizing an 8-year-old child's brain with an age-specific template (i.e., a template constructed based on 8-year-old children) and with an adult template.
The metric calculates the sum of the conditional entropy between each image in the group and a representative template image constructed iteratively using principal component analysis.
The sequences of the primer, template, and flap constructs for each forked DNA substrate are shown in Table 1.
Sequences for the primer, template, and flap constructs of all substrates used in this study are shown in Table 1.
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