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Control cDNA synthesis reactions to check for genomic DNA contamination during RT-qPCR consisted of the same template and components as the experimental reactions without the reverse transcriptase enzyme.
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In this second, overlapping PCR reaction, the overlapping PCR primer pairs of all the exons and the outmost primers (0.1 µM each) were pooled and used in a 25 µl reaction mixture containing 2 µl of the pooled template and the other components (see above).
To do so, we used a "linear template-matching procedure" that involves taking the average z-score of voxels falling within the template minus the average z-score of voxels outside the template and selecting the component in which this difference (the goodness-of-fit) was the greatest [10], [20], [21], [22].
Metal hydroxide modified carbon particles could play a role as hard template and simultaneously support metal components on the mesopores during the crystallization of zeolites.
For this, a master mix containing SYBR Green and other components (except template and primers) was used.
Δ Rn is defined as the difference between Rn+ (Rn of a reaction containing all the components including template) and Rn− (Rn of a no template control).
Telomerase, a ribonucleoprotein complex with RNA template and catalytic subunit hTERT core components, is able to add reiterated telomeric repeat sequences to the very ends of chromosomes.
In each set of data, maternal heart beats were identified by correlation to a maternal QRS signal template and the maternal PQRST signal components were then digitally averaged and subsequently subtracted from the signal traces.
It also takes as input modeling knowledge about the studied domain, represented as a library of template model components, i.e., entities and processes.
Complex DNA mixtures with low template (LT) components provide the most challenging cases to interpret and report.
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