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To assess the sizes of RNA transcripts that Pol IV is capable of making in vitro, as well as the possibility that Pol IV may initiate or terminate transcription with favored nucleotides, we used a 7249 nt single-stranded bacteriophage M13 genome as a DNA template and compared transcripts made by affinity-purified Pol IV or II.
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We then ran the preplay analysis as before estimating the number of preplay events for each down-sampled cued and uncued template and comparing them to each other and chance levels.
The presence of inhibitory substances in the sample matrix was assessed by spiking the samples with defined amounts of DNA template and comparing concentration thresholds between the matrix and controls, whose difference was always < 1.
We performed in vitro transcription on supercoiled and linear templates and compared the transcript yield (Figure 5).
Two homology models of P2Y14R based on either an agonist- or antagonist-bound structure of P2Y12R were generated as templates and compared for docking performance.
We found a new template grid from Shanghai Stock Exchange, and compared it with the classic one.
This information is obtained from a precomputed table of the template that is directly transformed and compared with the image table.
To estimate the quality of the template and to compare expression levels, we amplified mouse glyceraldehyde 3-phosphate dehydrogenase (GAPDH), a ubiquitously expressed abundant transcript with specific primers (5'-AACGGGAAGCCCATCACC and 5'-CAGCCTTGGCAGCACCA).
The model has been evaluated and compared with the main template through Ramachandran plots, Verify 3D and Protein Statistical Analysis (ProSA).
The report by Hart and Boone compared cervical and full spine graphs by creating a template and manually measuring and comparing one graph to another.
Template inhibition of the qPCR was tested by adding 2.17 × 10 plasmid copies containing fragment of the morphine-specific Fab gene from Mus musculus gene to reactions containing template DNA or cDNA and comparing the result to a dilution series of the plasmid as described in [ 3].
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