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Primers were tested in a conventional PCR using genomic DNA as the template and checked on an agarose gel to ensure that the primer PCRs resulted in a single band of predicted size.
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Data were extracted using an extraction template developed for this study, and checked for completeness and accuracy by two members of the research team (YK and BK).
Data were extracted against a data abstraction template by one author (AO) and checked by another (DNB) with reference to the full article text.
Non-template controls were included in each run and checked for no amplification.
Reads were aligned against a reference template, trimmed (keeping only > =100 bp sequences), and checked for chimeras.
The DNA templates were prepared using Montage Plasmid Miniprep96 kits, and checked by electrophoresis in 1% agarose gel.
40 In contrast, other states measure TRECs and check for DNA template integrity at the same time.
Compliance with the template is checked: deviation may lead to rejection of the dossier because of incompleteness and the conclusion of "no added benefit proven" for the drug under assessment.
The quality of the template was checked after purification by agarose gel stained with etidium bromide.
The reason for the low level of omissions in the prescription is because the prescription is created from a standard electronic template, initially checked by two separate radiation therapists, followed by the radiation oncologist at the time of providing final authorization.
To guarantee that the last step of this random walk can quickly arrive at the first Cα of the next template fragment, the distance l between the current Cα and the first Cα of the next template fragment is checked at each step of the random walk, and only walks with l < 3.54 n are allowed, where n is the number of remaining Cα-Cα bonds in the walk.
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CEO of Professional Science Editing for Scientists @ prosciediting.com