Sentence examples for template and both from inspiring English sources

Both HDA and RPA use proteins to facilitate the binding of primers to their respective template and both technologies enable multiplex amplification.

YFP fluorescence, confirmed by corresponding emission peaks recorded by the confocal laser scanning microscope, was only observed when the treatment involved the truncated yfp* repair template and both complementary gfp-TALEN units (Table S2 and Figure 2, A−C).

The standard reaction volume was 20  μl and contained 1 × SYBR Green PCR Master Mix ABII), 2.0  μl of cDNA template, and both forward and reverse primers at 10  μ M. The initial PCR denaturation step was performed for 5 min at 95°C, followed by 40 cycles of 60 s at 95°C (melting) and 60 s at 60°C (annealing/elongation).

The forward and reverse primers should be complementary to each other (i.e. anneal to the same location on opposite strands of the template) and both ends are either G or C. Adjust the length of the primers so that the estimated Tm is greater than or equal to 80°C.

Similarly, modellers may notice that a relationships is stronger at the beginning of the conflict (e.g., demographic precursors) so they would use the 'decaying impact' template and specify both the impact and rate of decay.

Denaturing agents DMSO and betaine (Sigma) were added to destabilize secondary structures in the template, and improve both the PCR and sequencing quality.

Sequence data obtained from the iMDA template and from both of the genomic templates covered more than 99.98% of the reference genome at least 1X coverage) and greater than 99.9% of the genome with at least 5X coverage.

Here, we present a facile technique to design self-supported 3D N-doped graphene foam (NGF) via simply annealing in chemical vapor deposition furnace which used powder metallurgy nickel template and melamine as both carbon and nitrogen source.

The molecular imprinted polymer (MIP) was prepared on the electrode surface using erythrosine (ERT) as the template molecule, and both m-dihydroxybenzene (m-DB) and o-phenylenediamine (o-PD) as functional monomers.

A T7 promotor primer (Agilent Low Input Quick Amp Labeling Kit) was then added and both RNA template and T7 primer were denatured at 65°C for exact 10 min before being cooled rapidly on ice for 5 min.

Rapid modernization undone by conservative revolt became both template and warning for Afghan progressives, "who have returned again and again to his unfinished project, only to succumb to their own blind spots".