Sentence examples for template and base from inspiring English sources
Like DNA and RNA polymerases, the SSU uses a nucleic acid template and base complementarity to direct synthesis of a polymeric product.
PolyBayes [ 62], a software designed to use genomic sequence as a template and base quality values to discern true allelic variations from sequencing errors, was used to reveal SNP polymorphisms.
Titanium silicalite (TS-1) was successfully synthesized by using tetra-propylammonium bromide (TPABr) and n-butylamine as the template and the base, respectively.
Some Y-Family members can bind an incoming dNTP even in the absence of a base pair partner (templating base) and base stacking with the primer end.
Libraries were double-indexed77 in 100 μl reactions using varied amounts of template and H2O based on library richness, 10 μl PfuTurbo buffer, 1 μl PfuTurbo (Agilent Technologies), 1 μl dNTP mix (25 mM), 1.5 μl BSA (10 mg/ml), and 2 μl of each indexing primer (10 μM).
Each subsequent 20 μL of PCR contained 50−100 ng template and was based on the primer pairs listed in Table S1 and standard PCR conditions.
These nanostructures have been generally synthesized through techniques like chemical vapor deposition, VLS method, lithography, template based and solution based approaches.
Fixed bases (maximum of six bases for short templates and twelve bases for long templates, Table S1 red) in the barcode region were used for filtering: barcodes which had at least one mismatched fixed base were removed.
Employing single-turnover kinetic methods, we examined human DNA polymerase β and its novel X-family homolog, human DNA polymerase λ, to determine which nucleotide and template base was preferred when encountering 8-oxodG and 8-oxodGTP, respectively.
3'-RACE PCR was carried out with appropriate template and degenerate primers based on N-terminal amino acid sequence of the target cDNA, UPM primer, or GCGT15 primer.
The mutagenicity of an adduct is also affected by its location within a DNA template and the neighboring bases.
