Sentence examples for template and appropriate from inspiring English sources

The deleted forms of PLSCR1 (see on Fig. 7) were amplified by PCR using pGAD-PLSCR1 as a template and appropriate primers.

For expression of the GST-SLPI W1 and GST-SLPI W2 fusions, PCR fragments obtained using pLex-SLPI as a template and appropriate primers were inserted into the EcoRI-XhoI sites of the pGEX4T-1 plasmid.

PCR was performed in a Mastercycler using HiFi polymerase (Qiagen; with supplied buffer), dNTP mixture, total DNA (or plasmid DNA) of P. aminophilus as the template and appropriate oligonucleotide primers (Additional file 20).

The encoding DNA fragments were amplified by polymerase chain reaction (PCR; Eppendorf mastercycler ep gradient thermal cycler) using genomic DNA (ColT) or plasmid DNA (ColG and H) as template and appropriate primer containing the restriction sites for subsequent cloning (Tables  2 and 3).

We examined the extent of replacement of wild-type copies of individual genes after each mutagenesis by PCR, using DNA isolated from wild-type and mutant cells as templates and appropriate forward and reverse primers (Fig.  1 and Supplementary Table S2).

The growth of vertically aligned carbon nanotubes has been successfully reported using a porous template and the appropriate amount of catalyst such that the pores can physically hold the nanotubes in the vertical direction and the bundles of nanotubes can support each other during growth.

Real-time quantitative RT-PCR was performed in a ChromoTM 4 (MJ Research, USA) using the SYBR Green RT-PCR KiTakaraara, China) and 10 μL reaction volumes, which contained 5 μL of SYBR Green PCR mix, 0.2 μM of each forward and reverse primer, 1 μL of diluted cDNA template, and the appropriate amount of sterile ddH2O to achieve the desired reaction volume.

Both the smart "self-template" effect and appropriate activation effect are crucial for the rational hierarchical porous structure.

By upscaling library preparation, using rRNA-depleted templates, and selecting appropriate barcodes, we were able to prepare libraries suitable for sequencing on Illumina's HiSeq-2000 platform while achieving excellent genome coverage in Arabidopsis root samples.

JNK1 fusion constructs, K55R substitution mutants, and V5 tag-containing mutants were created using the PrimeSTAR Mutagenesis Basal Kit (Takara Biomedical) with zebrafish JNK1 cDNA and pcDNA6/V5-His A expression vector (Invitrogen), or derivatives, as the templates and the appropriate combinations of the forward and reverse oligonucleotide primers.

Thus, for each of these resources, the query to obtain its class and appropriate template name has to be performed.