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Alternatively, repair of a ZFN-induced DSB by homologous recombination (HR) with an appropriately designed exogenous "donor template" (an approach known as "gene targeting") can be used to introduce a specific mutation near the break or to insert a DNA sequence at the the break.
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For all of the above reasons as well as the fact that we are working with very small amounts of material, we prefer single-tube preparation-to-amplification of both RNA and DNA templates, an approach made possible for the first time by the procedure described in this paper.
Using cl-N6 hRFC as a template, an analogous approach was used to insert single cysteine residues into TMD5 (positions 160, 161, 163, 164, 167, 168, 171, 172, 174 and 175).
The new software employs a template approach, where each template follows the same common steps in the workflow for design of formulated products, but has the option to employ different product specific property models, data and calculation routines, when necessary.
Qualitative results were analyzed by a team of experienced qualitative researchers using multiple styles of qualitative analyses, including a template approach and an editing approach.
TTF (test template framework [6]) is an approach that generates unit tests from Z specifications.
In the template approach, a landmark template or a set of landmark templates scan the image to identify the target landmark according to the strength of the template matching response.
Although our study was limited to mammary tumor in an animal model, it provided a template approach to perform similar studies with other tumor types using different radiotracers.
These guidelines represent the end product of an intense process and provide a template approach to the early resuscitation and support of patients with sepsis, based on a synthesis of evidence that has been shown to improve the outcome of the septic patient.
Nanotubes and nanowires were synthesized using template-directed electrochemical deposition, an approach pioneered by Martin [7, 8].
The analysis starts by a pitch estimation using a template matching approach as proposed by Goldstein et al. [33].
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