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Furthermore, the RT reaction using host RNA isolated from total blood cells of rabbit and mice as the template also did not produce amplification (lane 1 and 2 in Figure S5B), further confirming that the three novel miRNAs are specific for S. japonicum.
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Phyre-de-novo combines the output of both HHpred and Phyre2 and in case no good template identified, also does template-free modeling.
PCR reactions without template were also done as negative controls for each primer pair.
All of the template options that did not have a head CT item also did not have a nystagmus assessment item.
Note: we also did similar analysis for the U.S. participants (e.g., using age-appropriate Chinese or U.S. template), and found analogous findings.
Apple's concern over template-based apps for restaurants, Webb added, also doesn't make sense because "there's only so much you can do with apps that perform the same utility – ordering food".
They also do not take into account security and privacy issues of biometric templates stored in the database [20, 43].
As positive controls for exogenous primers, PCR was also done using the transfected plasmids (hOCT3/4, hSOX2, hKLF4 and hc-MYC) as templates.
The Simmons template also means never having to visit a locker room.
In common with the first study, the template conditions do also have an effect on the topics that are recorded in the questionnaires.
She also sent Mr. Collins a model settlement agreement that the attorney general's office used as a template for a variety of issues, and that template did not include confidentiality language.
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