30 Participants' individual functional images were projected onto a template cortical surface, after which a 6 mm full-width half-maximum (FWHM) smoothing kernel was applied to the surface data, and data were down sampled to 4 mm resolution.
These preparations often require removal of the templates after synthesis, which may damage the desired configurations of the hollow spheres.
Three microliters of purified DNA-free RNA was used as template for reverse transcription, after which the six cDNAs were amplified via 17 cycles of a long-distance PCR (LD-PCR) using the SMART™ PCR cDNA Synthesis Kit (Clontech, Mountain View, USA).
We used a single-subject template, the Johns Hopkins University Montreal Neurological Institute template, which was normalized to Montreal Neurological Institute space.
The cancelable template is converted into binary stream (s 1,s 2,s 3,…s L ), where L is the number of bits in cancelable template after the conversion, which is to be hidden into cover image using LSB steganography [35].
The variation in band intensity and disappearance of some bands may correlate with level of photoproducts in DNA template after genotoxical treatment, which can reduce the number of binding sites for Taq polymerase.
PEDOT was synthesized by VPP over a colloidal crystal thin film composed of monodisperse polystyrene colloids functionalized with a Fe(III) tosylate catalyst, after which the polystyrene template was selectively removed.
The PCR product was used to direct synthesis of dsRNA using T7 RNA polymerase (Invitrogen) after which the DNA template was removed by DNase digestion.
Finally, 16 myocardial VOIs were defined manually on parametric PTF images, according to the 16 segments model of the American Heart Association [ 16], after which this VOI template was projected onto the parametric PTI images.
The only template we have is Quebec, where a second referendum was lost by only about a point, after which support for independence went off a cliff".
