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There's a decent amount of flexibility despite Spotlight using a fairly common template across all projects, making it functional regardless of whether you've funded an iPhone accessory, a book of poetry or a live art installation.
Implementation of the template across all centres should have yielded a higher degree of completeness for some data sets.
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As target RNA, we used a serially known number of Armored HIV RNA molecules, and observed that 9 enzymes we tested were consistently sensitive to ∼1,000 copies, seven of which were sensitive to ∼100 copies, while only 5 were sensitive to ∼10 RNA template copies across all replicates tested.
Conversely, adjacent contigs will inherit the same template strands across all libraries.
Adjacent contigs will have a lower probability of an SCE between them and a higher chance of inheriting the same template strands across all the libraries compared with contigs at opposite ends of the chromosome, which will be far more likely to have an SCE event between them.
The maximum contribution attributed to the UV-induced damage template was small across all SAMMs, the highest value being 13.2 % in DO45169, from the LIRI-JP_icgc dataset.
Lesions were then manually traced by a neuropsychologist (K.E.B). on individual slices of the patient's brain overlaid on to the standard template, with crosschecking across all three planes.
The concentration of parasite DNA in the template was standardized across all samples to avoid the potential technical bias of scoring more heterozygous loci in samples with high parasite densities.
The template will be generalisable across all types of exercise interventions.
The template should be generalisable across all types of exercise interventions for any conditions.
All of these interactions are also present in at least one of the templates used to build the model, although none of them is conserved across all templates.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com