Sentence examples for template abundance from inspiring English sources

Relative template abundance was quantified using the relative standard curve method described in the ABI PRISM 7900HT manual and the data were normalized for the quantity of the β-actin transcript [69].

Thus, read number can reliably be assumed to approximate relative template abundance.

The correlation between sequencing depth and template abundance is insufficient to precisely arrange mutations within these groups.

Low Ct values indicate the presence of higher template abundance, and are usually associated with a higher reproducibility and lower variability [ 22].

Since CT values are directly indicative of original target template abundance, we have created four categories into which most qPCR targets seem to fit, namely: 1).

Each experiment was performed in triplicate and the average cycle threshold (Ct) value was used as a measurement of initial template abundance.

Misincorporation might take place as a result of the balance between synthesis and excision that compromises the rate of DNA extension, both of which can be influenced by template sequence, abundance of dNTP pools and their bias (Bebenek and Kunkel 1990, 2000).

In this work, we present a five-layer microfluidic system for parallelized, rapid, quantitative analysis of RNA templates with low abundance at the single-cell level.

It is possible that the nested PCR that we performed, increasing selectively the abundance of template DNA, reduce this problem.

As a constitutive control, a 217-bp fragment of 16S rRNA was amplified using the same PCR conditions but with only 1% of the cDNA synthesis reaction as template due to the high abundance of 16S rRNA in total RNA extracts.

Local abundance of repair template may direct the repair toward HDR instead of NHEJ.