Sentence examples for template a second from inspiring English sources

However, assuming that replication is broadly similar to modern systems (proceeding 5' → 3', and reading 3' → 5' from a template), a second strand will always be synthesized as complementary to a template.

A secondary nested PCR was carried out for RNA samples from G. ternezi, D. rerio, P. annectens, and A. baerii species (see Table 1) using as template a first PCR reaction, obtained using the primers [AR3S × AR3AS], at a 1/100 dilution and a second set of nested degenerate primers [AR.AS × AR.AAS] or [AR.Bs × AR.AAS].

Briefly, using pUHC-131-1 pUHC-131-1 pUHC-131-1 luciferase and SK-eGFP as template for the eGFP coding region, two PCR fragments were synthesized that served as template for a second PCR reaction delivering the final orf.

A 2.5 μl aliquot of the first PCR was then used as template for a second PCR.

Serial array capture was performed over two copies of each array using the enriched fraction from the first round of capture as a template for a second round.

Plasmids were isolated and used as a template for a second PCR with primers designed for cloning into the pEHISTEV vector (NcoI/HindIII), in order to add the His-TEV tag at the N-terminus of the protein.

The second proposed mechanism, known as semiconservative replication is when each strand of the parental DNA acts as a template for a second newly-synthesized complementary strand, which then combines with the original strand to form a DNA molecule.

The resulting amplicon was used as template in a second PCR reaction with primers zz-for_2 and zz-rev.

The two first PCR products were used as template for a second PCR reaction in which the two external primers 040101 and 040102 were used.

An aliquot (3 µl) of this reaction was used as template for a second round of amplifications (35 cycles) using primers 5'-CACCCATAAGCCTCGAATATGTGG-3' and 5'-TAGAATAAATCAGAA GTACAGTGTCC-3'.

In a nested PCR, the initial PCR reaction is performed on the extract and the product of this first reaction is then used as a template for a second PCR reaction.