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A transparent stack comprising a glass slide, a polydimethylsiloxane layer attached to the glass and polycarbonate (PC) as an external film is mounted on a micromanipulator positioned under an optical microscope with a temperature-controlled stage.
In this study, a temperature-controlled stage was created in order to investigate the effect of cell temperature on cytoplasmic streaming, and the cells of an aquatic plant (Egeria densa) were used.
As a result, he might suggest outfitting a system with a temperature-controlled stage like that available from Linkam Scientific Instruments in Surrey, United Kingdom.
HeLa cells in a ΔT35 dish (Bioptechs) were infected with rSeVLeGFP for 18 h, then the movement of LeGFP was recorded using a Leica DMIRB inverted fluorescence microscope equipped with Image-Pro Plusoftwarere (Mediacybernetics) while maintaining the cells at 37°C on a ΔTC3 temperature-controlled stage.
Cells were imaged at a temperature-controlled stage set at 37 °C.
Cells were imaged on a Zeiss confocal microscopy system equipped with a temperature-controlled stage.
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The prototype calorimeter consists of a cryostat with two temperature-controlled stages and a base stage that are operated at nominal temperatures of 8 K.
Cells were mounted on a temperature controlled stage (37°C) and infected with S. Typhimurium in the presence of 30 µg/ml chloramphenicol.
Brains were frozen on a temperature-controlled freezing stage, coronally sectioned (25 µm) on a sliding microtome, and stored in a solution of PBS containing 0.02% NaN3 at 4°C.
In future assays, it may be useful to grow worms and conduct rapid upshifts in a room maintained at 15°C by use of a temperature-controlled microscope stage, in order to bypass mounting embryos at room temperature (which would likely allow one to further clarify the "Unclear" determinations in Table 4).
Next, the multiwell plate is transferred to a temperature-controlled microscope stage for microinjection.
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CEO of Professional Science Editing for Scientists @ prosciediting.com