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This temperature was held for 2 min.
Subsequently, the temperature was held at 4°C.
The final column temperature was held for 20 min.
The electrolyte temperature was held constant at 35 °C.
The ion source temperature was held at 250°C.
During bioreactor cultivation temperature was held constant at 30 °C.
The temperature was held at 250°C for 10 min before cooling down to 40°C.
The drying temperature was held at 70°C to avoid glucose caramelization [23, 33, 34].
The temperature was held at the peak temperature for 60, 90, 120 min, respectively.
At 60 °C, the temperature was held constant for 10 min.
For all steps, the substrate temperature was held at 500°C.
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