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The combination of both temperature upshift and physiological osmolarity promoted induction of protein synthesis (Figure 2A).
The influence of the temperature upshift, from 30°C to 37°C, on promoter activity was also assessed.
Sph2 is expressed under routine in vitro conditions and temperature upshift to 37°C [87], [88], [89].
Furthermore, DegP and FkpA can be induced to express by temperature upshift via the periplasm specific σE heat-shock regulon [33].
Thus, we decided to evaluate another combination of conditions, the effect of the urine pH plus the physiological osmolarity and the temperature upshift.
Additionally, PlipL41 was induced to a similar extent by the temperature upshift and the combination of physiological osmolarity and temperature (Figure 4A versus 5A).
Similar(20)
The temperature sensitive mutants were maintained in a 15°C incubator and shifted to a 26°C incubator to perform temperature upshifts for determining embryonic lethality.
We performed temperature upshifts 20 hr before axotomy and examined axon regrowth at 6 hr post axotomy (hpa).
Western analysis of protein extracts of several rhizobia species confirmed an increase of the amount of sHPS with temperature upshifts.
Upon lowering the melt temperature, the upshift of SANS profile observed only below a critical scattering vector is consistent with the signature of nucleation and growth during LLPS in the metastable region.
Group I and II: Many environmental factors have been identified inducing a VBNC state in bacteria, which are natural stress, temperature stress, osmotic upshift, oxygen stress, white light, pasteurization, and chlorination (Oliver 2005).
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