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Thermal cycler machine was extensively used machine for temperature transfer of polymerase chain reaction (PCR) to amplify DNA sample.
We show that HTS analysis of soil eukaryotes was robust to environmental variation, e.g. rainfall and temperature, transfer effects, storage effects and spatial variation.
Further, the influence of the tool size on the parameters temperature transfer times and temperature homogeneity was examined by introducing a scaling factor of the forming tool.
This is imputable to the fact that as the electric current increases with pulse on-time, machined material will melt with the most amount of high temperature transfer to the machined surface (Kumar et al. 2013).
Results show that higher ambient temperature contributes to faster temperature transfer but lower tensile properties, and the effect of material parameters variation should be considered especially under elevated temperature.
The proposed microfluidic approach emerge as an engaging tool for processes intensification, since it provides better mass and temperature transfer than conventional methods with a reduction not only of the size and energy consumption, but also of by-products and reagents consumption.
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It is then allowed to cool at room temperature, transferred to a polythene bottle and stored in a refrigerator.
After the required time, the mixture was cooled rapidly to room temperature, transferred to a separating funnel and the upper phase (model oil) was withdrawn.
All Bio-Rad® Criterion™ 12% SDS-PAGE gels were run at room temperature; transfers were continuously cooled to 4°C with a circulating cooling-coil.
To prepare the working solution, a certain volume of each stock solution was allowed to reach room temperature, transferred into a new glass vial resulting in a mixture 1∶1∶1∶1 (v/v/v/v) and vortexed.
The mixture is brought back to room temperature, transferred on ice and neutralized with concentrated perchloric acid.
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