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In this study hot mix asphalt (HMA) creep compliance master curves were constructed using different temperature shifting techniques and fitting functions.
Several temperature shifting techniques have been proposed for construction of relaxation modulus master curve of asphalt mixes using the time temperature superposition principal; however, it is not clear which one works better than the others.
Therefore, the main objective of this study was to compare the relative ability of five common temperature shifting techniques, i.e. Numerical, Log-Linear, Williams-Landel-Ferry (WLF), Modified Kaelble, and Arrhenius, to construct the relaxation modulus master curve for dense graded asphalt mixtures.
Internalization of radiolabeled ligand was examined by temperature shifting to 37°C for varying times from 30 to 120 mins.
We chose these media as it has been shown that temperature shifting and decreased nutrient load are effective tools to resuscitate certain VBNC bacteria [12], [28].
Aarts' group used flow cytometry to examine internalization, whereas we used more traditional methods employing radiolabeled ligand, temperature shifting, and acid washing.
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An increase in temperature shifts the curve to the right (decreased affinity; enhanced release of oxygen); a decrease in temperature shifts the curve to the left (increased affinity).
The melting curves were normalized and temperature shifted to allow the direct comparison of samples.
There is only limited proteomic analysis of suspension-grown CHO cells describing a direct comparison of temperature shifted versus non-temperature shifted cultures using 2D-DIGE.
The melting curves were normalised and temperature shifted to allow samples to be directly compared.
Temperature shifts trigger differential gene expression and stage transformation in Leishmania[ 32].
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