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The second connection is the one between the high temperature shield and a second shield called low temperature shield, which has a temperature during reactor operation around 150 °C.
The first one is the connection between module and the first part of a shield, called high temperature shield, which operates at a temperature around 500 °C, close to that of the blanket module.
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This heating strategy may be viewed for high temperature shielding or minimization of thermal runaway for temperature sensitive applications, such as environmental control system, chemical storage reservoirs, etc., where heat flow is controlled by solid wall resistance.
To achieve a multi-year liquid helium lifetime and to avoid exceeding the limited capacity of the JT cooler, very strict requirements are placed on every source of heat leak into these surfaces from the higher temperature shields.
After extensive washing with PBS, p58 antibody was detected with Alexa Fluor® 568-conjugated anti-mouse IgG (1 µg/ml; Invitrogen) for 20 min at room temperature shielded from light.
After additional rinses, wells were incubated with the chromogen solution for 30 minutes at room temperature, shielded from light.
Each section was mounted on a gelatine-coated slide and allowed to dry at room temperature, shielded from light.
The substrate solution was prepared as close as possible to the time of use and stored briefly at room temperature, shielded from light.
The T g is the globe temperature measured by a sensor placed inside a hollow, black-painted metal globe and T a is the air temperature shielded from radiation.
For 7-AAD staining, we added 20 μL of 7-AAD solution (BD Pharmingen) to 400 μL of cell suspension and incubated it for 10 min at room temperature, shielded from light.
Cells were then incubated with Guava Cell Cycle Reagent (Millipore) for 30 min, at room temperature, shielded from light, and analyzed using the Guava easyCyte 5HT Flow Cytometer (Millipore).
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