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In addition, a comprehensive temperature protocol for the analysis based on the Beer Lambert Law remains elusive.
Stimulated primary human umbilical vein endothelial cells (HUVECs) were exposed to a dynamic temperature protocol analogous to clinical settings.
An inter-comparison study was conducted based on fine particulate matter samples collected during summer in Beijing to investigate the influence of the thermal optical temperature protocol on the OC to EC ratio.
Here, empirical response-surface modeling was used to show how the temperature protocol in TOT analysis can be modified to distinguish pyrolyzed OC from BC based on the Beer Lambert Law.
Though the temperature protocol had substantial influence on the OC to EC ratio, the contributions of secondary organic carbon (SOC) to OC, which were predicted by the EC-tracer method, did not differ significantly among the five protocols.
Therefore, our restrictive temperature protocol for olfactory learning consisted of a 30 min pre-incubation at 37°C, followed by three 2.5 min training cycles and a 5 min test period, all at 34°C (Figure 3A).
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Although the % accuracy of stability QC samples which were stored in ice-cold water bath (protocol-A) was higher than that of room temperature (protocol-B), it was not in the acceptable limits.
Depending on the molecular weight (MW, Table 1) of the ligands, SA was done, using two temperature protocols, up to 50 °C (MW ≤ 300) or 80 °C (MW ≥ 300).
b The response of eight spinal trigeminal neurons at baseline and after incremental additions of NOX-C89 0.05 5 mg/kg (mean ± sem) during the temperature protocols shown in panel A. c Average activity during the whole period of elevated temperature was increased above baseline.
Primers and temperature protocols used have been published previously [50], [51], [52] with the exception of IL-10.
This replicates commonly reported time and temperature protocols [ 2, 19– 23].
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