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Advanced refolding strategies by pulsed refolding and temperature leap, favoring the native pathway of specific model proteins were also implemented.
Direct refolding kinetics approximated by a folding kinetic of first order and aggregation kinetic of second order was similar to batch processes, while pulse and temperature leap refolding yields were higher than direct batch refolding method.
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The temperature-leap tactic described above was used to generate functional proteins for CD measurements.
We previously have used the temperature-leap tactic to promote and maintain properly folded protein structure [44], [45].
Stripping down, I threw myself in the water, registered its near-freezing temperature and leaped straight back out.
To achieve the objective, FLC membership functions are optimized for minimizing the operational cost of the HRES based on weekly and daily prediction of data for grid electricity price, electrical load, and environmental parameters, including wind speed, solar irradiation, and ambient temperature, using shuffled frog leap algorithm.
Leap Wireless (nasdaq: LEAP).
Britain's icy desolation ended abruptly 14,700 years ago when there was a dramatic leap in temperatures across the globe according to ice-cores found in Greenland and lake sediments in Germany.
And, although the exact rise in that proportion varied from basin to basin, all of them saw a significant increase.What caused that increase is, of course, debatable and since the second-largest percentage increase was in the Southwest Pacific, where no significant temperature rise was observed, leaping on changes in sea-surface temperature as the sole cause might be premature.
Stone says that building a large-enough oven that could be controlled at high temperatures was a technological leap.
"I think even the hard-core climate people are looking at this and saying: 'What on Earth'?" a director at the U.N.'s World Meteorological Organization, said of the leap in temperatures.
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