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The variations in temperature induction by ASA are further illustrated in Figure 1G.
The activity of cells obtained by temperature induction was higher than that obtained by enzymatic digestion.
To achieve high levels of soluble form of recombinant human growth hormone (rhGH) we used suitable host strain, appropriate induction temperature, induction time and culture media composition.
To accomplish this improvement in titer, factors such as strain compatibility, carbon source, temperature, induction point, and inoculation ratio were initially optimized.
The factors that are known to affect the protein solubility include plasmid copy number, culture media, bacterial strain, induction temperature, induction time, and concentration of the inducer [28],[29].
Furthermore, while no crystalline copper oxide could be highlighted for copper nanoparticles electrodeposited at room temperature, induction heating leads to the formation of a crystalline Cu2O shell that could have interesting catalytic properties, among others.
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The optimum predicted cultivation conditions for the maximum expression of extracellular recombinant CGTase were found to be comprised of: 20 °C post-induction temperature, induction-starting time when cell optical density is 0.3 at 600 nm, 1.0 mM xylose, 50 μM IPTG and 29 h post-induction time, with a predicted extracellular recombinant CGTase activity of 9144.28 U/ml.
Moreover, the circadian clock participates in cold signaling pathways in Arabidopsis by gating the low-temperature induction of CBFs (CRT/DRE, C-repeat/drought-responsive, binding factor) and modulating low-temperature Ca2+ signals [35], [36].
A control strain containing the empty vector was grown with continuous heat-shock or without temperature-induction.
These differences are particularly relevant because the pat1 temperature-induction system in haploids is frequently used to model meiosis in fission yeast.
The formation temperature, maximum induction temperature and induction time are measured.
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