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The reaction solution was allowed to stir at room temperature for approximately 4 h.
After that, the autoclave was cooled to room temperature for approximately 70 min.
Regarding the stability of the produced derivatives, both were found to be stable at room temperature for approximately 2 hour.
The treated samples and standards were allowed to stand at room temperature for approximately 40 min prior to analysis.
The snus tins were stored at 4°C until use and allowed to equilibrate to room temperature for approximately 15 min before use.
All specimens were stored at − 30 °C until testing, at which point they were thawed at room temperature for approximately 24 h.
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Prior to ultrasonic measurements the samples were allowed to equilibrate to the desired temperature (25°C) for approximately 20 minutes.
BCIP/NBT substrate (Pierce Chemical, Rockford, IL, USA) was added and the blot was developed at room temperature (RT) for approximately 10 minutes for alkaline phosphatase secondary antibody.
Bottles were incubated instream under ambient light and temperature conditions for approximately 2 h, after which a subsample from each bottle was filtered as for POC above.
Lymphocytes were separated from culture media by centrifugation at 200 g and then placed in 0.075 mol/L KCl at room temperature (RT) for approximately 10 minutes.
One or two rosette leaves of 4- to 5-week-old plants were blended in a blender with deionized water at room temperature (RT) for approximately 30 s.
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