Sentence examples for temperature fixation from inspiring English sources

The capture was improved by taking slide temperature, fixation temperature and fixation duration into account.

After 9 days of culture, cells grown to confluence in three 150-mm plates were fixed in situ with 1% formaldehyde for 10 min at room temperature, fixation was then blocked with 125 mM glycine, cells were rinsed and scrapped in PBS.

An equal volume of 4% paraformaldehyde was added (final concentration of 2%) and cells were incubated an additional 10 minutes at room temperature for fixation.

Then fixed cells were stained by biotinylated CTB or biotinylated antibody (anti-CD59 or anti-CD71) at the same temperature as fixation for 20 min. After 3× washes with 5% FBS in PBS, streptavidin-conjugated QD655 dyes were incubated with the cells at corresponding temperature for 20 min, followed by 3× washes with 5% FBS in PBS.

Thus, it appears that shorter time and lower temperature of fixation can significantly affect FFPE sample quality.

After incubation, cells were washed three times with HMEM and promptly imaged at room temperature without fixation.

Live pre-pupal wings were dissected in Schneider's medium and incubated with antibody against V5 at 0°C followed by washing and chasing at room temperature before fixation.

In brief, cells were fixed using 1% formaldehyde for 10 min at room temperature and fixation quenched with 150 mM glycine.

Caulobacter cells grown overnight in liquid PYE were rinsed in PBS buffer and resuspended in 4% paraformaldehyde (Sigma-Aldrich) solution for 1 hr at room temperature for fixation.

The success of capture depends on a number of tissue-slide adhesion factors such as the type of slides used or the method and temperature of fixation [ 19].

For immunofluorescent staining, cells were permeabilized in 0.1% (v/v) Triton-X (Sigma-Aldrich), supplemented with 5% donkey serum (Sigma-Aldrich) for 30 min at room temperature after fixation in 4% paraformaldehyde (Sigma-Aldrich).