After crystallization of the product, it was then cooled to room temperature, filtered, and washed with deionized water.
The digested mixture was allowed to cool to room temperature, filtered and then made up to 100 mL with de-ionize water.
The reaction mixture was stirred for a period of 2 days at room temperature, filtered by celites 545, to collect a crystal clear solution.
The reaction mixture was cooled to room temperature, filtered and the filtrate was concentrated in vacuo.
The reaction mixture was cooled to room temperature, filtered through Celite®, using EtOAc as an eluent.
The solution was stirred overnight at room temperature, filtered, and the solvent was removed under vacuum.
The preparation was infused for 8 min and then was cooled to room temperature, filtered, and evaporated to complete dryness.
The extract was cooled at room temperature, filtered and evaporated under reduced pressure in rotary evaporator to Sonchus asper methanolic extract (SAME).
To this suspension was added diisopropylethylamin (DIPEA) (385 μl, 2.25 mmol) and the mixture was swirled for 16 hours at ambient temperature, filtered, and washed repeatedly with DMF.
The reaction mixture was heated at 80 °C for 20 min under microwave then cooled to room temperature, filtered through a Celite pad, and the filtrate concentrated in vacuo.
