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Apart from temperature, cultivation pH also influences spore characteristics.
The area of improving recombinant protein production by the use of reduced temperature cultivation has been documented by several investigators [ 32, 38].
For low temperature cultivation, TGMS-Co27 and H1493 plants were germinated on November 15, grown on November 18 and transplanted on December 12 in a paddy field at Lingshui (18.48°N; 110.02°E), Hainan province.
For high temperature cultivation, TGMS-Co27 and H1493 plants were germinated on May 5, grown on May 8 and transplanted on June 3 in an experimental field at Wuhan University Institute of Genetics (Wuhan, China; 30.54°N, 114.36°E).
One area in which there is currently a large volume of interest with regard to improving recombinant protein production in mammalian cells is through the use of lower temperature cultivation [ 12].
As low temperature cultivation has been reported to increase the surface expression of some membrane proteins, as well as the production of some non-membrane associated proteins [ 14, 5, 13], we looked at the effect of this treatment on wild type hERG expression.
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Similarly, iIN800 was able to highlight the importance of unsaturated fatty acids when comparing high and low temperature cultivations (Table 6), which is known to be important for maintaining proper membrane structure and fluidity [ 40].
Finally, the MW of HA polymer was further increased to 2.5 MDa by low-temperature cultivation (26 °C) which reduced cell growth inhibition.
Clearance of the plasmid was accomplished with high-temperature cultivation (37 39 °C) for 2 3 days, followed by replica plating on selective and nonselective plates to confirm restoration of apramycin sensitivity.
odr-3(n1605) and eat-16(nj8) mutants migrated towards warmer and colder temperatures than the cultivation temperature, respectively.
The algal broth temperature during cultivation was 25 °C ± 2 °C.
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