Exact(2)
We sampled live animals at day 1, 5, 9 and 11 representing young, young-mid, mid- and late-life animals, avoiding transgenerational contamination by utilizing the temperature conditional sterile strain TJ1060 [ spe-9 hc88) spe-9 hc88b26)II].
Furthermore, the mutant strain was indistinguishable from wild type with respect to growth at either 25 or 35°C, eliminating the possibility that the mutant is temperature conditional (data not shown) [ 15].
Similar(58)
Disruption of the ERV1 gene or null mutation in the haploid strain led to temperature-conditional mutation resulting in alterations of the mitochondrial inner membrane, complete loss of mitochondrial genome, arrest in cell-division cycle, and death of the yeast after a few replication cycles [ 24, 40, 42].
Pioneering studies of the secretory pathway established that cell division is blocked at the non-permissive temperature of conditional secretion-defective mutants of S. cerevisiae [26].
Thus, YBP2 has a temperature-sensitive conditional synthetic-lethal interaction with spindle checkpoint genes.
Consistent with these observations, ybp2Δ exhibited temperature-sensitive conditional synthetic-lethal interactions with ame1-4 or okp1-5, respectively (Figure 5G, bottom).
The hopTum-l mutants provide a convenient system to test this, as the mutation is temperature-sensitive conditional.
Synthetic genetic interactions were used to construct an essential synthetic genetic network using temperature-sensitive conditional alleles.
Importantly, iGFPi and deGradFP permit a temperature-dependent conditional knockdown of gene function that mimics a severe loss of function in specific cells or tissues in most instances.
Furthermore, mutants of Escherichia coli K12 which were defective in phosphatidylserine synthetase, were isolated as temperature-sensitive, conditional lethals [ 42]. * kdtA: 3-deoxy-D-manno-octulosonic-acid transferase is the enzyme of kdtA.
Equations are derived for temperature dependence of conditional equilibrium constants for disproportionation reactions of ThCl2 in LiCl, NaCl, NaCl + KCl and CsCl, ThBr2 in NaBr + KBr, and ThI2 in NaI + KI.
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