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The reaction mixture was stirred for 1 h at room temperature, concentrated under reduced pressure and dissolved in ethyl acetate.
After cooling to room temperature concentrated aquaous NaOH was added until the formation of a white precipitate was observed.
After addition of 0.23 g NaBH OAc 3 (1.07 mmol, 1 eq), the mixture was stirred for 1 h at room temperature, concentrated under reduced pressure and dissolved in ethyl acetate.
The pellet was solubilized in 70% formic acid for 1 h at room temperature, concentrated under vacuum and diluited 1∶10 in 2 M Trizma and then 1∶3 in H2O before assessing protein concentration by BCA Protein Assay Kit (Pierce) and performing the Aβ1-42 quantification by ELISA assay.
The resulting solution was cooled to room temperature, concentrated in vacuo, and 10%% aqueous hydrochloric acid solution was added.
The mixture was heated under reflux for 2 h, cooled to room temperature, concentrated in vacuo, and water (100 mL) was added.
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