Also expression kinetics of Mss4 mRNA and protein were much alike the temperature challenge curves.
At that time, they were subjected to the temperature challenge for QTL analysis.
Individuals were starved for 48 h prior to the temperature challenge.
More recent results suggesting that specific mechanisms retard ageing in response to lower temperature challenge thermodynamic explanations.
Environmental temperature challenge has a direct impact on ectothermic marine animals, and influences biological functions at all levels, from molecules to whole organisms [ 2, 3].
This work will serve as a starting point for studying the transcriptomic response of hydrothermal mussels and annelids in future experiments in response to thermal stress at various conditions of duration and temperature challenge.
Also, we conducted an otherwise identical control experiment, where a subset of the clones (6 robust, 6 brittle) were allowed to evolve using a periodic temperature challenge of the standard 25°C environment.
The total time from initiation of the temperature challenge until the 50 temperature tolerant individuals were sampled from all three pairs of half-sib families was approximately 8 h.
