Sentence examples for temperature agar from inspiring English sources

Cells (1×103) were then cultured in DMEM plus 15% FBS in 0.35% (w/v) low melting temperature agar between layers of 0.7% low melting temperature agar.

For anchorage-independent growth assays, stable transfectants were seeded onto 6-well plates on 0.4% agar gel in appropriate medium supplemented with 20% FCS, over a bottom layer of 0.6% low-melting temperature agar gel.

Retinas were isolated, embedded in a low-gelling temperature agar, and 200 µm slices were made on a vibrating microtome (Leica VT-1000S).

The fixed E17.5 embryos were immersed in PBS containing 4% Gadolinium ("ProHance" gadoteridol by Bracco Diagnostics) for 3 days, after which they were embedded in high melting temperature agar containing 4% Gd, and then imaged with MRI, using a T2-weighted, 3D fast-spin echo sequence.

The E13.5 embryos were then embedded in 1% low melting temperature agar which was subsequently dehydrated in methanol and cleared in BABB (a 2 1 mixture of Benzyl-Benzoate:Benzyl-Alcohol) prior to being imaged with OPT using auto-fluorescence mode [ 17].

Cells were plated on six-well plates (500 per well) (Corning, Corning, NY), with a bottom layer of 0.7%% low-melting-temperature agar in DMEM and a top layer of 0.25 % agar in DMEM.

Water levels in the chamber were adjusted so that the window in the cranium remained slightly above the water surface, and then 3 5% low-gelling-temperature agar (Nacalai Tesque, Tokyo, Japan) in saline was poured into the cranial cavity to stabilise the electrode that was subsequently inserted into the corpus cerebelli.

Edges and any papillary muscles present were trimmed to enable flattening of the excised tissue before gluing it endocardium-down (histoacryl tissue adhesive; Braun, Melsungen, Germany) onto a block of 4% agar (low melting-temperature agar; Nusieve GTG agarose; Lonza), which in turn had been fixed on top of the vibratome cutting stage.

The most suitable carbon source, nitrogen source, and culture temperature were agar, yeast extract, and 25 °C, respectively, for agarase production by one-factor-at-a-time design.

After solidification at room temperature, the agar block was removed and transferred to a round petri dish, where it was kept in 1x imaging solution until use, when it was cut into approximately 1 cm broad slices.

The melting and freezing temperatures for agar are 85°C and 40°C, respectively.