The high activity of the catalyst is complemented by its high sedimentation ability; thus, it usually takes a few minutes for the polymer to precipitate from the alcohol solution when cooled below the phase-transition temperature after the completion of the reaction.
The temperature was immediately quenched down to the room temperature after the completion of each growth.
This is because, in the phase-hardened alloy, the γ-phase fully transforms into the ε-phase on cooling to room temperature after the completion of the reverse ε-γ transformation.
Patients were observed for at least 2 h for blood pressure, pulse, temperature and allergic reactions after completion of the infusion.
The reaction mixture was stirred at room temperature for 10 min. After completion of the reaction (monitored by TLC), the reaction mixture was diluted with CH2Cl2 (20 mL), and the organic layer was then washed 2 times with 5 mL of saturated aqueous NaHCO3.
After completion of dehydration, the temperature is raised to the value needed for silica reduction.
After completion of the low temperature treatment, the plants were returned to the growth chamber (8 °C/16°C/16 h 26 °C day/night) and flowering time was recorded.
After completion, upon cooling to ambient temperature, the conversion was directly determined by GC MS analyses.
After completion of extraction, the solvent was completely removed by vacuum drying at low temperature (<50°C).
After completion of the amplification, samples were subjected to a temperature ramp (from the annealing temperature to 95°C with a transition rate of 0.1°C/s) with continuous fluorescence monitoring for melting curve analysis.
After completion, the reaction mixture was cooled to room temperature and then diluted tenfold with N,N-dimethylformamide.
