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Suppressive subtractive hybridization (SSH) and quantitative reverse transcription PCR (qRT-PCR) were performed on RNA isolated from mature (stage V) oocytes of a temperate population of Ae. albopictus.
At day 21 pi, the temperate population of BUE depicted the lowest TE (Chi square test: p < 0.05).
Known and unknown tropical host and pathogen systems are likely to shift their ranges closer to subtropical and temperate population centers as climate changes at higher latitudes.
For these reasons we introduced in the experiment as a control a temperate population derived from a natural population sampled in Bologna on October 1991.
One temperate population came from Draveil, near Paris (48°44N, collected in 2002), where the average temperature oscillates from 3°C in January to 20°C in July; another temperate population came from Bologna (44°30N, collected in 2002) where the average temperature oscillates from 4°C in January to 24°C in July.
The first is a temperate population that comes from Draveil, near Paris (48°44'N, collected in 2002); the second temperate but warm adapted population comes from Athens, Georgia-USA (33°57'N, collected in 2002).
Similar(50)
In temperate populations of Aedes albopictus, exposure to short-day lengths causes the adult female to oviposit diapausing eggs.
By contrast, most tropical and temperate populations conformed to a negative exponential distribution, consistent with frequent regeneration.
QRT-PCR experiments were performed for 53 transcripts using a novel experimental design that included replicate temperate populations that do undergo photoperiodic diapause and replicate tropical populations that do not undergo a photoperiodic diapause.
These were selected from pools and populations of highly variable germplasm, including landraces from all over Latin America, and some germplasm from temperate populations mixed in as well.
In addition, the phenomenon of cold-hardiness in temperate populations [15], which might increase the ability of Ae. albopictus eggs to survive in areas with low winter temperatures, should be investigated.
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