Sentence examples for tel buffer from inspiring English sources
Beads were washed for 3 minutes with 1 ml of each of the following buffers in succession: LSW buffer, HSW buffer (20 mM Tris-HCl pH7.5, 1 mM EDTA, 1% Triton X-100, 0.1% SDS, 500 mM NaCl) and TEL buffer (0.25 M LiCl, 1% NP-40, 1% sodium deoxycholate, 1 mM EDTA, 10 mM Tris-HCl pH8.1), followed by two final washes with TE buffer (10 mM Tris-HCl pH8.0, 1 mM EDTA).
For each of the 151 isolates, 1 to 4 colonies of a pure culture grown on BA for 18 h were resuspended in 100 μL of TEL-buffer containing 10 m M Tris/HCl and 10 m M EDTA, pH = 8.5.
The human telomere base sequence (Tel-22) prepared in Na+ buffer forms a G-quadruplex structure with the characteristic CD spectrum shown in Figure 1A.
DNA from the neoadjuvant chemotherapy study samples was isolated from fresh-frozen tumour tissue, using RNAzol (Tel-test) and Back Extraction Buffer (consisting of guanidine thiocyanate, sodium citrate, and Tris).
In contrast, this same Tel-22 G-quadruplex prepared in K+ buffer reveals a markedly different CD spectrum as shown in Figure 1B with a positive ellipticity at 295 nm but with a broad shoulder at 265 nm.
The G-quadruplex structures and stabilities were characterized by CD spectroscopy to demonstrate that the 22-nucleotide sequence (Tel-22) was folded into G-quadruplex structures under the Na+ and K+ buffer conditions that were used and exhibited thermal stabilities and folding topologies comparable to those previously published.
The Tel-22 (K+) G-quadruplex exhibited a Tm of 68 °C in 100 mM KCl buffer and 0.01 M Tris (pH 8.0), unfolding at a temperature approximately 10 °C higher than the unfolding temperature of its Na+ counterpart.
The melting temperatures, Tm, were derived from the midpoint of these transitions and are shown in Figure 2. The Na+ form of the Tel-22-mer G-quadruplex exhibits a Tm of 59 °C in 100 mM NaCl and 0.01 M Tris buffer (pH 8).
