Water (2 mL) and HNO3 (2 mL) were added to the samples before the tubes were covered with Teflon caps, transferred to a Teflon rack in the microwave system which then was closed.
This work provides a comprehensive explanation of why ink can be transferred onto Teflon, a material that has lower surface free energy than ink, and thus of the principles behind ink transfer in the offset printing process.
After 30 min, a black liquid was transferred to Teflon lined autoclave of 100 mL capacity.
Cells were extracted by adding 3 7 ml of nitric acid and transferred to Teflon tubes to be digested in a MarsXpress microwave (Method EPA 3051A, 2007).
After that it was transferred into Teflon lined sealed stainless steel autoclave and maintained at 150 °C for 12 h under autogenous pressure.
The procedures were described as follows [29]: 20 mM Cu(CH3COO 2 and 10 mM thiourea were dissolved in diethylene glycol (DEG) sequentially and transferred to Teflon autoclave.
Briefly, 0.5 g of OS-EL was accurately weighed and transferred to Teflon vessel.
Cells were first concentrated via centrifugation, rinsed several times with Milli Q water and 1 m m EDTA to remove weakly sorbed metals, transferred to Teflon Savillex vials, and digested in ultrapure HNO3 and HF.
The mixtures were stirred within 60 s to form homogeneous pastes, transferred into Teflon molds (Φ 10 mm × 20 mm) and then stored at 37°C for 24 h; finally the hardened 10% Mg/CSH composite, 20% Mg/CSH composite, and pure CSH cements were obtained.
In order to obtain monocyte-derived macrophages, another part of the peripheral blood suspension was transferred to Teflon flasks (NalgeNunc, Rochester, USA), supplemented with 10% autologous canine serum and 20% DE GMCSF (granulocyte-macrophage colony-stimulating factor), and cultured at 37°C with 5% CO2 (Forma Scientific Incubator, Waltham, USA).
