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Each piezometer was equipped with its own dedicated teflon tubing assembly, which included a 1 3 ft long teflon plug to prevent freezing in winter.
The other end of the segment was tied, 10 ml of DMEM alone (control) or DMEM containing E. coli F18 was inoculated, and the segment was sealed with a Teflon plug (5 mm in diameter).
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After each sample collection, the Au traps were re-plugged with a Teflon stopper, wrapped with Teflon tape, double-bagged in the zipped plastic bags, and sent to the U.S. Geological Survey (USGS Mercury Research Laboratoryy (Middleton, Wisconsin) via express package delivery for Hg analysis.
High-volume air samplers fitted with polyurethane foam plugs and Teflon filters were used to collect particulate-and gas-phase samples of ambient air.
Immediately after solvent injection, the whole assembly was removed from the stand and swung by hand using the string for 1 min. After the manual centrifugation, the collection trap was removed from the NMR tube, and the opening of the tube was plugged with a Teflon ball (Bruker BioSpin).
Replace pump and skimmer baskets, pressure gauges, pool ladders, diving boards etc. Use thread sealant such as Teflon tape on all threaded plugs, connections.
Although CO2 must be generated in the first T-shape mixer, neither bubbles nor gas plug flow was observed in the Teflon tubes.
All vessels were connected with silicone (VWR) and Teflon (Nalgene) tubing and vented through ports (Chemglass) plugged with glass wool.
The PFA tube was plugged into the groove to construct the Teflon mold).
A Teflon® piston with a tight O-ring seal was fitted into the glass tubing from the top end, with a plug of buffered 1% agarose gel inserted between the piston and the surfactant solution that was added through a stainless steel port from the other end of the sample chamber.
The resulting agar plugs were extracted with 1 mL of the TB medium and a glass tissue homogenizer with a Teflon plunger (VWR).
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