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After 10 min, cells in Ficoll or dextran were mixed on a vortexer at maximum speed for 2 to 3 min; cells in PEG could be broken in the same way or by ∼50 gentle hand strokes in a 2 ml glass homogeniser with a teflon piston (Wheaton), until >95% of the cells had released their nucleus as seen by phase-contrast microscopy.
Two Teflon piston prostheses of different diameters (0.4 and 0.6 mm) were used successively.
After that, the tissue was transferred into a Potter-Elvejhem homogenizer with a Teflon piston and homogenized for a second time on the ice.
Similar(57)
A Teflon® piston with a tight O-ring seal was fitted into the glass tubing from the top end, with a plug of buffered 1% agarose gel inserted between the piston and the surfactant solution that was added through a stainless steel port from the other end of the sample chamber.
At delivery, the midwife drew blood from the umbilical cord by heparinized syringes with Teflon-lined pistons.
To avoid window contamination by the lubricating oil, self-lubricant Teflon-Bronze composite piston rings were used in the optical section.
Frozen tissue samples were homogenized (150 mg/ml) in a tissue homogenization buffer [250 mM sucrose, 20 mM Tris base and protease inhibitors (Sigma #8340 protease inhibitor cocktail; 10 μl per millilitre buffer)] using a glass vessel and piston-type Teflon pestle.
The device consisted of a teflon cylindrical chamber with a piston oscillating vertically and driving a working fluid back and forth between a heat source and a cold heat exchanger.
It consists of cylindrical Teflon beaker with oscillating displacement piston and two electrodes for streaming potential measurement.
In addition, a thin layer of Teflon was placed at the end of the piston to accommodate lateral slip of the sample assembly during deformation.
The piston rod (PR) terminated with three 1 mm thick Teflon orifice plates, which formed the damper piston.
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